奶牛酮病的血浆代谢组学分析

doi:10.11843/j.issn.0366-6964.2013.10.022

Abstract

Abstract:

Through the application of biological NMR technique combines principal component analysis (PCA) and orthogonal partial least-squares discriminant analysis (OPLS-DA), changes of endogenous metabolites with clinical and subclinical ketosis cows were analyzed, which may help to study the effects of ketosis on cow metabolic process, and find the differences metabolites. The plasma metabolic profiles of the three groups were obtained by ¹H nuclear magnetic resonance (¹H NMR). Differences in metabolites were acquired by analyzing the collected data with mode analysis. Results were as follows: compared with the healthy control group, 23 different metabolites were obtained in subclinical (13 were up-regulated, 10 were down-regulated) and clinical (6 were up-regulated, 17 were down-regulated) ketosis groups. By comparison, 28 different metabolites were obtained between clinical ketosis group (4 were up-regulated, 24 were down-regulated) and subclinical ketosis group. The results indicate that ¹H NMR spectra combined with pattern recognition technique can effectively get the different metabolites of diagnosing clinical and subclinical ketosis, and panoramic reveal that widely metabolic disorder on cows in the process of ketosis, which lays the foundation for the exploration of ketosis mechanism and obtaining new biomarkers in the future.

CLC Number:

S856.5

SUN Ling-wei, ZHANG Hong-you, XIA Cheng, BAO Kai, LI Lan, LI Ying. Serum Metabonomic Studies of Ketosis in Dairy Cows[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(10): 1667-1674.

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Serum Metabonomic Studies of Ketosis in Dairy Cows

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